Composite

Part:BBa_K1740003

Designed by: Keisuke Sugahara   Group: iGEM15_KAIT_Japan   (2015-09-06)

pLac+RBS+145N+stop codon

The coding sequence Dronpa145N contained in the part: BBa_K1740000 doesn't contain the stop codon to the terminus. In that part, it doesn't contain a promoter sequence and a ribosomal binding site(RBS) sequence necessary for the protein expression. Therefore, we designed this part which added to Lac promoter(pLac), RBS and double stop codon to Dronpa 145N.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 899
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None